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| Registros recuperados: 33 | |
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| Montagna,D.R.; Rigo,V; Ramayo,L.G.; Goldman,L.H.; Huguet,M.J.; Maceira,N; Jar,A.M.. |
| El objetivo de este trabajo fue caracterizar las inmunoglobulinas séricas y la IgY de la yema de huevo de ñandú, a través de las técnicas de electroforesis en acetato de celulosa, electroforesis en gel de poliacrilamida y Western-blot. Se estudiaron 19 ñandúes clínicamente sanos de 14 a 15 meses de edad, provenientes un criadero de Balcarce, Argentina. La electroforesis en acetato de celulosa de los sueros mostró la presencia de cuatro fracciones proteicas: albúmina, alfa, beta y gamma globulinas. La concentración de las gamma globulinas fue de 0,69 ± 0,18 g/dl, con un rango de 0,42-1,05 g/dl. La corrida electroforética de la IgY muestró que esta se ubica en la fracción gammaglobulínica. La electroforesis en gel de poliacrilamida evidenció, tanto en suero... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ãandú; Electroforesis; SDS-PAGE. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200002 |
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| Liang,Hongxia; Liu,Yao; Zhu,Jun; Guan,Peng; Li,Shuangcheng; Wang,Shiquan; Zheng,Aiping; Liu,Huainian; Li,Ping. |
| Sichuan basin, situated in the west of China, is the fourth biggest basin in China. In order to describe a systematic study of the cry2-type genes resources from Bacillus thuringiensis strains of Sichuan basin, a total of 791 Bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. The method of PCR-restriction fragment length polymorphism (PCR-RFLP) was used to identify the type of cry2 genes. The results showed that 322 Bacillus thuringiensis strains harbored cry2-type genes and four different RFLP patterns were found. The combination of cry2Aa/cry2Ab genes was the most frequent (90.4%), followed by cry2Aa (6.8%) and cry2Ab alone (2.5%), and only one novel type of cry2 gene was cloned from one isolate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus thuringiensis; PCR-RFLP; SDS-PAGE; Novel cry2-type gene. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100018 |
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| Scarcelli,Eliana; Costa,Elizabeth Oliveira da; Genovez,Margareth Élide; Cardoso,Maristela Vasconcellos; Bach,Erna Elizabeth; Torres,Ana Paula. |
| Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; SDS-PAGE; Biotyping. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400006 |
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| De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G. |
| Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host... |
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Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382 |
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| Deef,Lamiaa Elsayed Mokhtar. |
| ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425 |
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| Puccia,R.; Juliano,M.A.; Juliano,L.; Travassos,L.R.; Carmona,A.K.. |
| We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents of the basement membrane in vitro. In the present study, we separated the components of P. brasiliensis culture filtrates by electrophoresis and demonstrated that the serine-thiol exocellular proteinase has a diffuse and heterogeneous migration by SDS-PAGE, localizing in a region between 69 and 43 kDa. The hydrolytic activity was demonstrable after SDS-PAGE using buffered agarose overlays of Abz-MKALTLQ-EDDnp, following incubation at 37oC, and detection of fluorescent bands with a UV transilluminator.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P. brasiliensis; Serine-thiol proteinase; SDS-PAGE; Fluorescent-quenched peptides. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500019 |
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| Yüzbasioglu,Ertugrul; Dadandi,Mehmet Yasar; Özcan,Servet. |
| In this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard’s similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed... |
| Tipo: Journal article |
Palavras-chave: Phlomis; Phlomoides; Phlomis taxa; SDS-PAGE; Seed proteins. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200002 |
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| Silva,André Luis Coelho da; Caruso,Cecília Sulzbacher; Moreira,Renato de Azevedo; Horta,Ana Cecília Góes. |
| Cotyledon explants were first cultured on MS medium supplemented with 4.52 M 2,4-D and 0.46 mM kinetin. The development of the calli was followed (0, 4, 8, 12, 16, 20, 24, 28 and 32 days after) and the growth curve was determined, based in fresh and dry weight. The growth curve presented sigmoidal form with four distinct phases. The highest growth percentage was observed at the exponential phase and the lowest at the stationary phase. These results indicated that cotyledon callus subculture should be performed 20 days after inoculation. The calli obtained after a period of 28 days were freeze dried, macerated and submitted to extraction with buffers of different pH values (2.6; 4.0; 6.0; 8.0 and 10.0) and the proteins in the extracts were determined by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine; Leguminosae; Callogenesis; SDS-PAGE. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000600009 |
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| MEDICI,LEONARDO O.; GONÇALVES,FABÍOLA V.; FONSECA,MARCOS PAULO S. DA; GAZIOLA,SALETE A.; SCHMIDT,DAIANA; AZEVEDO,RICARDO A.; PIMENTEL,CARLOS. |
| ABSTRACT In this study, we are presenting recommendations to the best agricultural use as well as for plant breeding of three millet cultivars namely ENA1 and ENA2, which have African origin, and BRS1501 originally from India. These cultivars were evaluated for growth, yield and grain quality traits. The morphological traits evaluated in this study indicated that the African genotypes ENA1 and ENA2 are better than the Indian genotype BRS1501 for no-till farming or to produce forage with 15% of crude protein at flowering and at harvest to produce stover (around 7% of crude protein content) for livestock feeding. The BRS1501 cultivar exhibited the highest values for total crude protein, albumins and prolamins, phytate and mineral contents in grains. ENA1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mineral density; SDS-PAGE; Phytate; Seed storage proteins; Amino acids. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401749 |
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| Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A.. |
| Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008 |
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| Chacur,M.G.M.; Martins,E.A.F.; Silva,A.A.; Guaberto,L.M.; Gabriel Filho,L.R.A.; Sanches,O.C.; Oba,E.. |
| Objetivou-se estudar a morfometria corpórea, as características do sêmen, o perfil proteico do plasma seminal em SDS-PAGE e a concentração sérica de testosterona em cervos-sambar (Cervus unicolor), criados em cativeiro, na estação reprodutiva da primavera. Quatro machos com idades entre 12 e 36 meses foram avaliados em quatro momentos, com intervalos de sete dias, com peso corpóreo (60,5 a 89,0kg), índice de massa corporal (93,07kg/m2 a 126,56kg/m2), volume do ejaculado (0,50±0,35mL a 0,75±0,28mL), motilidade espermática (87,75±4,78% a 90,00±7,07%), defeitos totais (17,25±5,81% a 47,72±17,55%), testosterona sérica (6,43±4,33ng/dL a 166,00±64,48ng/dL) e proteínas do plasma seminal com bandas entre 7,6 e 142kDa. As características dos ejaculados não... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervidae; Cativeiro; Sêmen; SDS-PAGE; Andrógeno. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501392 |
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| Registros recuperados: 33 | |
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